1.
LC GC North America
; 38(6):320-324, 2020.
Article
in English
| ProQuest Central | ID: covidwho-20243314
ABSTRACT
Aside from evaporation of water from buffer solutions, precipitation of buffer salts can also easily occur when a buffer solution comes into contact with a pure organic solvent. [...]one should avoid- at all costs-a situation where a LC system component containing an aqueous buffer is flushed immediately with a pure organic solvent. Solvents and Buffers Figure 1 shows a picture of a buffer bottle I observed in an LC laboratory about a year ago. Many of the aqueous buffer solutions used in LC (and even high-performance liquid chromatography [HPLC] grade water, if given enough time and exposure to aboratory dust) are environments quite favorable to microbes, particularly those in the middle of the pH range.